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To determine the latter, methods that stain the terminal boutons are used.
Most synaptic boutons in the mammalian central nervous system are too small to be investigated directly with electrophysiological tools on the single synapse level.
There is an estimated total of 30000-50000 boutons of different types across the entire surface.
In this case, spillover is limited to the postsynaptic sites under a single synaptic bouton , and occupancy is likely to vary across sites.
When postsynaptic receptors are saturated, a quantum of synaptic current corresponds to the response evoked in a single synaptic bouton .
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