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The oxidant tertiary butylated hydroperoxide could effectively induce TNF- [alpha] mRNA expression.
The dried analytes are butylated by the addition of butanol - HCI and 65 degree C incubation.
Butanolic HCl (3 mol/L) was then added, and the mixture was butylated for 10 min at 70 °C and dried at 70 °C under reduced pressure.
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