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Cholesterol was dissolved in ethanol and distilled, deionized water was added dropwise until a white precipitate appeared.
Between fertilizer applications, the pots were kept moist with acidified, deionized water.
The resulting solution was made up to 100 ml with deionized water.
The last residues were washed with deionized water until the washes reached pH 5.5.
All the reagents were of the highest quality available and deionized double-distilled water was used throughout the experiments.
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