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The gels were stained with Coomassie blue, destained , and dried, then analyzed by autoradiography overnight at - 80 deg C.
Gels were stained in ethidium bromide, destained in water, and photographed over a UV transilluminator.
Papanicolaou staining and destaining do not interfere with indirect IS-PCR for HPV DNA detection.
We stained the gels in 0.1% Coomassie blue for 1 h and destained them in a 40% methanol and 10% acetic acid solution overnight.
The gel was stained with ethidium bromide for 30 min and subsequently destained for 3 hr.
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