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Crosses were done between transgenic plants carrying pShortDs and plants containing transposase to analyze the ends of extrachromosomal linear Ds elements.
Although it is possible that these extra ND5-like sequences exist as extrachromosomal , episomal DNA, several lines of evidence suggest they are of nuclear origin.
Circular extrachromosomal Ac and Ds molecules have been described previously, although their origin and possible relation to the circular molecules discussed here is unclear.
Upon heat shock during larval development, FLP and I-SceI produce the extrachromosomal targeting molecule.
In this study, I investigated genetic requirements and DNA substrates for DSB repair in S. pombe using a new simple extrachromosomal DSB repair assay.
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