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Ammonium molybdate was added to all reactions to inhibit activity from non-specific phosphatases.
The ready restoration of biosynthesis by removal of tungstate suggests that the molybdate is weakly held by the enzyme and that xanthoxal is its substrate.
As yet no gene responsible for a Mo transporter has been identified, although there is some evidence that molybdate may be taken up by the phosphate and/or sulphate transporter.
It is noteworthy that the values obtained for the wild type in cells grown in the presence of molybdate are lower for nitrate reductase and nicotinate hydroxylase.
‘Even if a system is known to have minimal leakage, regular testing for nitrite or molybdate is recommended,’ he says.
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