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We have been examining the potential use of oligonucleotides to direct nucleotide exchanges in episomal and chromosomal genes.
The proper orientation and the sequence of the cloned oligonucleotides were checked by nucleotide sequencing.
For single bulges in RNA oligonucleotides , the conformation usually depends on the nature of the flanking base pairs.
Intermolecular tetrameric complexes are usually only formed by short oligonucleotides that contain single tracts of contiguous guanines.
This problem would be enhanced using arrays composed of long oligonucleotides since only RNAs whose protected regions overlap with the oligo sequence would be identified.
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