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Male frogs were anesthetized in tricaine methanesulfonate and then were decapitated and pithed .
Animals were pithed through the right orbit to the spinal column with a round copper rod of 1.5 mm in diameter.
Part of the traditional butchering practice involved a technique known as pithing , where a hole was made in the animal's skull.
Cattle are humanely stunned with a captive bolt stunner that penetrates or piths the brain rendering the animal unable to feel pain.
Adult frogs were anaesthetized in 15% ethanol, then killed by pithing .
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