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saponify
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The purpose of an alkali is to saponify the oils and dislodge ordinary soil and other matter.
Most coolants and lubricants used in machining operations today are saponifiable -- that is, they can be made soluble by uniting with alkali to form soap, which is rinsed from parts relatively easily.
The ethyl ester protecting groups of the tartrate were saponified in 1 M NaOH and heated at 40-45 deg C for I h.
After visualization with primuline (0 • 01% in 80% acetone), lipid bands were scraped off, saponified and fatty acids methylated with boron trifluoride.
A sample of the isolated anthocyanins was also saponified using the method described by Durst and Wrolstad to remove acylation.
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