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We illustrate our approach through application to data from a study of mutation induction in lacI transgenic mice after exposure to the flame retardant tris (2,3-dibromopropyl) phosphate.
The flame retardant, tris (2,3-dibromopropyl)phosphate (tris-BP), which is a mutagen and causes cancer and sterility in animals is absorbed from fabric by people.
Nonspecific sites were blocked with blocking solution containing 20 mM tris base, 150 mM sodium chloride, 3% nonfat milk, and 0.3% Tween 20 for 1 h at room temperature.
Another flame retardant, tris -(1,3-dichloro-2-propyl)-phosphate, and its expected metabolites are mutagens.
After transferring the membranes were blocked with 3 per cent bovine serum albumin in tris buffered saline and 0.1 per cent tween 20 and incubated with patients' sera followed by IgM / IgG (Sigma, USA).
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