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To measure parasite burden of winners and losers, we assessed the number of eugregarine trophozoites in a male's mid-gut by removing the entire gut carefully and making light microscope thin-section preparations.
Ingested cysts hatch into trophozoites in the small intestine and continue moving down the digestive tract to the colon.
The cyst wall disintegrates in the small intestine, releasing motile trophozoites .
Biopsy of colonic ulcer edges may yield intramural trophozoites but carries with it the risk of perforation.
Exposure to bile is the primary stimulus for encystation, where trophozoites transform into cysts that pass out with the feces.
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