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The procedure for the isolation of PHU from freshly collected male urine included filtration, ultrafiltration and reverse-phase chromatography.
The supernatant was desalted by centrifugal gel filtration and concentrated by ultrafiltration as previously described.
The procedure for the preparation of CDA-II from male urine includes filtration, ultrafiltration and reverse-phase chromatography.
The ultrafiltration molecular weight separation transforms the product into 50 percent protein, allowing smaller molecular weight particles like minerals and lactose to pass through a screen while the protein is retained.
Specific processing by microfiltration, ion exchange and ultrafiltration can concentrate GMP in whey protein to 70 to 80 percent.
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