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In a confocal microscope, light passes through a pinhole to eliminate photons from outside the image plane.
Here we present the application of confocal spot detection combined with fluorescence fluctuation analysis.
The images were recorded in a confocal microscope.
The component whose diffusion is to be observed must be tagged with a fluorophore so that it can be imaged in the confocal microscope.
The boundary could be inspected during the experiment from the transmission image produced by the confocal microscope.
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