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Harris hematoxylin was the counterstain for the remainder of the antibody reactions.
The shape of the nucleus was visualized using a DNA counterstain in all experiments.
Immunoreactivity was visualized using diaminobenzidine as the chromogen, with Harris modified hematoxylin as the counterstain .
Dapi was used as the chromosome counterstain for fluorescent specimens.
No counterstain was used on the sections, but routine coverslips were applied.
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