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This problem would be enhanced using arrays composed of long oligonucleotides since only RNAs whose protected regions overlap with the oligo sequence would be identified.
The cDNA synthesis was performed at 42° for 30 min using 1 g of total RNA and an oligo primer.
Magnetic beads coated with streptavidin were then added and allowed to interact with the biotin on the oligo (dT).
The oligo triggers the actual gene repair process.
The oligo primer was end labeled using T4 polynucleotide kinase.
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