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The chromosomes were stained with acetic orcein and visualized with a light microscope.
Chromosomes were counted in root tips stained with acetic orcein .
The anthers of each flower were squashed in a drop of 2% acetic orcein to intensify the staining of meiotic cells.
After air drying, the cells were stained with 2% acetic orcein .
The slide was immersed in 1% orcein in 45% acetic acid for 15 min, washed in distilled water, air-dried and a coverslip was mounted in Histomount (National Diagnostics, Hull, UK).
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