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If all rotamers clash with the rigid part of the protein, additional conformers are made by 300 rotations around the final bond.
It is consistent with emission from different rotamers of the cyanine dyes formed immediately after excitation (vide supra).
In this study we use the dead-end elimination method to determine the possible rotamers of tryptophan.
Using broader Ï-values may result in broader distributions of rotamers , so we have avoided interpreting rotamer distributions that may be influenced in this manner.
The effects of the membrane are clearly illustrated by the increased frequencies of hydrogen-bonding rotamers and the snorkeling of Trp and Tyr out of the membrane.
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