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The sonicate was cleared by centrifugation at 12,000 g for 30 minutes at 4°C.
The sonicate was centrifuged for 10 min at 16,000 RCF and the resulting supernatant collected.
Finally, the sonicate in the third cuvette was additionally treated with DNase for another 1 h at 25 deg C, then Cyan 40 was added and the emission spectrum was recorded.
The sonicate was cleared by microcentrifugation for 5 min at 4° and mixed with the anti-HA.11 antibody coupled to Sepharose beads (prewashed three times with lysis buffer).
The samples were sonicated for 12 sec and cell number was analyzed using a Coulter counter.
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