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Peroxidases are present in the crease tissue at this time and it has been suggested that they may have a role in the formation of lignin / suberin in the chalazal cell walls.
Chemically, apoplastic barriers of roots are depositions of the biopolymers lignin and suberin within the cell wall matrix, which may occlude wall pores previously filled with water.
Histochemical staining showed lignin and suberin to be absent in both untreated and oleocellosis-damaged tissue.
Peroxidase is probably implicated in lignin and/or suberin formation during the polymerization of monoglinols or aromatic monomers.
Cytochemistry and autofluorescence observations were used to detect the presence of lignin and suberin in unfixed tissues.
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