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Samples were then centrifuged (17,000 × g for 10 min), and supernatant fluid was stored at - 20°C for VFA analysis.
The resultant supernatant fraction was used for the determination of lipid peroxidation and glutathione.
The tubes were centrifuged at 5000 rpm for 2 min, and the supernatant discarded.
The supernatant fluid was filtered and centrifuged.
Ammonia N was determined on the supernatant fluid using the phenol-hypochlorite procedure.
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