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This stage is usually considered to be a characteristic of fresh, or cryopreserved tissues, because other processing methods (fresh-frozen or freeze-dried) are considered to have already caused cell death in donor tissues.
Tissue was cryopreserved as soon as possible after surgical excision and stored in liquid nitrogen vapor.
Yeast can be frozen and revived, allowing samples from each population to be cryopreserved at intervals, and then competed against their ancestors to provide precise and reproducible estimates of fitness.
Moreover, tissue architecture is often compromised and background levels are higher in cryopreserved tissues, making immunohistochemical interpretation difficult if expression levels are low.
several major problems hinder attempts to cryopreserve large mammalian organs
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