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The number of colonies of the three plates was added and this number was used to determine the ratio of each mutant to the wild-type control within the homogenate from each animal.
Therefore, we used Western analysis to determine the relative contents of these contractile proteins in whole lung homogenates .
Free amino compounds were extracted from tissue homogenates according to the procedure described by Shaul and Galili.
The spectra of the two homogenates of the same tissue type from the biopsy samples of each patient were averaged to obtain a single spectrum.
Plasma membrane vesicles were purified from root homogenates using the sucrose gradient or two-phase partitioning procedure as previously described.
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