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The sonicate was cleared by centrifugation at 12,000 g for 30 minutes at 4°C.
Finally, the sonicate in the third cuvette was additionally treated with DNase for another 1 h at 25 deg C, then Cyan 40 was added and the emission spectrum was recorded.
The sonicate was centrifuged for 10 min at 16,000 RCF and the resulting supernatant collected.
The sonicate was cleared by microcentrifugation for 5 min at 4° and mixed with the anti-HA.11 antibody coupled to Sepharose beads (prewashed three times with lysis buffer).
The sample was sonicated to clarity in a sonication bath (Ultrawave).
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