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Seeds are soaked overnight in water then treated with tetrazolium to give an indication of viable, abnormal, and dead seeds in the seed lot.
This technique is more reliable than tetrazolium chloride for this species, because some deeply dormant viable seeds show minimal staining with tetrazolium .
The stains acridine orange, ethidium bromide, azur II, eosin B, trypan blue, Giemsa solution and nitroblue tetrazolium were purchased from Sigma.
After 24 and 48 h, aliquots were transferred to 96-well plates and incubated for 3 h with a tetrazolium salt.
The standard procedure for this assay was to remove the cell culture medium and add fresh media containing the MTS tetrazolium reagent.
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