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It must be remembered that the deuterated compounds have a lower Tm than the nondeuterated compounds.
For 2 H-NMR experiments, each sample contained 10 mg of deuterated lipid.
Quadrupole splittings of the dominant spectral feature were considerably smaller than the value of - 40 kHz expected for deuterated alanine methyl groups on immobilized peptides.
The activation energy is greater and the rate of the reaction is correspondingly less for the deuterated compounds.
They then dissolved the sample in deuterated acetone and filtered it before running the NMR spectra.
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