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PUVA-damaged and undamaged DNA was transfected into a yeast strain containing the ADE2 gene regulated by a p53-responsive promoter.
Stably transfected cells were selected with 500 g/ml G418 (Sigma).
Cells were transfected with eYFP-C10HRas 3-4 days before the experiment.
Schneider cells were seeded onto cover slips and after 24 hr the cells were transfected with plasmid pBSHS-Fl d.
I have purified, cloned, sub-cloned, transfected , blotted and detected DNA.
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