приклади
Nonspecific sites were blocked with blocking solution containing 20 mM tris base, 150 mM sodium chloride, 3% nonfat milk, and 0.3% Tween 20 for 1 h at room temperature.
Four times PCV of buffer I (10 mM tris , 1 mM ethylenediamine-tetraacetic acid [EDTA] and 5 mM dithiothreitol [DTT]) was added and the mixture was incubated on ice for 20 min.
After transferring the membranes were blocked with 3 per cent bovine serum albumin in tris buffered saline and 0.1 per cent tween 20 and incubated with patients' sera followed by IgM / IgG (Sigma, USA).
We illustrate our approach through application to data from a study of mutation induction in lacI transgenic mice after exposure to the flame retardant tris (2,3-dibromopropyl) phosphate.
Another flame retardant, tris -(1,3-dichloro-2-propyl)-phosphate, and its expected metabolites are mutagens.
Other